Real time pcr abi prism 7500 psc 563, nsc 338 real time pcr stepone psc 563 every time you use any of the qpcr instruments, you must sign the log book. Realtime pcr abi prism 7500 psc 563, nsc 338 realtime pcr. Gene expression with steponeplus realtime pcr biocompare. The intuitive software and protocol wizards help guide new users through their real time pcr experiments. Applied biosystems realtime pcr software user bulletinfor. Wait for the systems to start up, this may take up to 5 minutes. Quantitative realtime pcr qpcr is a type of reverse. Obtain the installer for the windows 7compatible realtime pcr software see supported equipment and software on page 2. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. This easy to use instrument comes with a builtin lcd touchscreen controllers allowing you to preconfigure experiments and save them in the system internal memory bank. Provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture. Applied biosystems stepone and steponeplus realtime pcr systems. Steponeplus realtime pcr system thermo fisher scientific. The can be set up in a variety of configurations and come ready to use out of the box, with intuitive data analysis and instrument control software.
Applied biosystems stepone qpcr comes with its own software for template input and data output but this is only for pc. The stepone realtime pcr system can be used via a pc, with a pc connected to a local area network lan, or as a standalone instrument pcfree. The stepone 48 well and steponeplus 96 well realtime pcr systems. Applied biosystems stepone qpcr comes with its own software for template input. Realtime pcr systems is perfect for both firsttime and experienced users. The abi stepone plus realtime pcr offers four color optical recording allowing for faster measurement.
Steponeplus 96well realtime pcr system school of medicine. Currently, qpcr experiments are becoming increasingly complexinvolving an expansive and growing list of targets from a larger number of samples, all with more technical replicates. Amplify sample with the steponeplus real time system. The steponeplus realtime pcr system is a 96well real time pcr instrument perfect for both first time and experienced users. One of the perks about the open qpcr interface is how simple and quick it is to create complex experiments. The abi stepone plus real time pcr offers four color optical recording allowing for faster measurement. Used real time pcr abi stepone the lab world group. Applied biosystems stepone realtime pcr system, laptop. Onestep realtime rtpcr is therefore generally less sensitive than twostep rtpcr. Install the software procedures for upgrading to the microsoft windows 7 operating system perform the windows 7 upgrade 4 applied biosystems realtime pcr software for research use only.
Utilizing robust led based 4color optical recording, the steponeplus realtime pcr system is designed to deliver precise. This easytouse kit enables users to calibrate the steponeplus realtime pcr system quickly and easily. Description the steponeplus realtime pcr system is a 96well realtime pcr instrument perfect for both firsttime and experienced users. Connect the usb drive to one of the usb ports on your computer. Applied biosystems stepone and steponeplus realtime pcr systems remarkably simple systems. Qiagen s realtime pcr cycler, the rotorgene q, combines multiple optimized design features to provide the outstanding performance and reliable results that your research demands. Stepone and steponeplus realtime pcr system thermo fisher.
Steponeplus realtime pcr system from thermo fisher scientific. For our lab we are considering the purchase of a new real time pcr machine. Each stepone realtime pcr system is factorycalibrated for optical and thermal accuracy, so simply remarkable realtime pcr results can be obtained right out of the box. Applied biosystems stepone and steponeplus realtime pcr systems getting started guide for vii presenceabsence experiments preface how to use this guide about the system documentation the guides listed below are shipped with the applied biosystems stepone and steponeplus realtime pcr systems stepone and steponeplus systems. Focused application software analyzes and interprets experimental results. Transfer the template file from your computer to a usb drive.
The steponeplus realtime pcr system is a 96well realtime pcr instrument perfect for both firsttime and experienced users. Applied biosystems steponetm and steponeplustm realtime pcr systems site preparation guide 7 preface obtaining information from the help system the stepone software has a help system that d escribes how to use each feature of. Real time pcr cycler and high resolution melt analyzer with 5 channels green, yellow, orange, red, crimson plus hrm channel, laptop computer, software, accessories. Steponeplus real time pcr systems getting started guide for genotyping experiments explains how to perform experiments on the stepone and steponeplus systems. Applied biosystems steponeplus realtime pcr system spectral. This remarkably simple real time pcr system is designed with an lcd touchscreen and a userfriendly interface that brings the power of genetic studies to researchers new to real time pcr fig 1. Prior to downloading any software to a computer used for instrument. Applied biosystems steponeplus realtime pcr system. The steponeplus realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. The smartchip realtime pcr system is a complete highthroughput. The stepone and steponeplus realtime pcr systems are covered by one or more of us patents nos. Applied biosystems stepone steponeplus realtime pcr systems. The steponeplus realtime pcr system is a 96well realtime. Applied biosystems latest introduction to realtime pcr, the steponeplus realtime pcr system, makes it simple and easy to get publicationready highquality realtime pcr results on day one.
Is there a maccompatible control software for the stepone rt pcr. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast and standard runs, and are configured for pcfree or. Youre now ready to explore the simply remarkable features of these systems. Realtime pcr qpcr is a powerful technique for genotyping and gene expression analysis. Laboratory exercise realtime pcr qpcr primer design using. Watch the video, find your best fit, or request a quote. Applied biosystems stepone and steponeplus realtime pcr.
Flexible and easy to use, these systems can be set up and operated with total confidence, even by researchers who have little or no previous realtime pcr experience. Getting started this is the initial screen youll see once logged in. Applied biosystems steponeplus for software version 2. Step up to high performance realtime pcr remarkably simple systems applied biosystems, a leader in technology solutions for life science research, introduces its latest innovations in realtime pcrthe stepone 48well and steponeplus 96well realtime pcr systems. A tutorial, using example experiment data provided with the applied biosystems stepone real time pcr software stepone software. These factorycalibrated one step systems make it easy to step up to highperformance qpcr. Jan 26, 2016 used real time pcr abi stepone the lab world group.
Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers. The applied biosystems stepone and steponeplus real time pcr systems offer intuitive software, fast and standard runs, and are configured for pcfree or networked operation. These remarkably simple realtime pcr systems are speci. Fixing software setup mistakes in real time pcr steponeplus.
Amplify sample with the steponeplus real time system qpcr. The applied biosystems steponeplus realtime pcr system spectral calibration kit contains one background plate and two preloaded, 96well fast optical reaction plates containing seven dye standards. Stepone and steponeplus realtime pcr system thermo. A tutorial, using example experiment data provided with the applied biosystems stepone realtime pcr software stepone software.
Stepone stepone realtime pcr system, laptop laptop. This easy to use instrument comes with a builtin lcd. The stepone software included with the steponeplus realtime pcr system runs on the windows xp operating system and provides instrument control. The power switch at the bottom left of the back of the instrument. The steponeplus real time pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software.
Apr 06, 2011 the steponeplus realtime pcr system can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Fixing software setup mistakes in realtime pcr steponeplus. A 96well realtime pcr instrument perfect for both firsttime and experienced users. In this 5 nuclease assay real time pcr qpcr kit, one step real time rt pcr can be performed in a single tube, simplifying amplification and minimizing the risk of contamination. The stepone 48 well and steponeplus 96 well realtime pcr systems are remarkably simple to use.
Steponeplus realtime pcr systems getting started guide for genotyping experiments explains how to perform experiments on the stepone and steponeplus systems. Intuitive data analysis and instrument control software that performs for a wide. Stepone plus real time pcr system basic operation 1. Is there a maccompatible control software for the stepone rtpcr. Amplified products are monitored in real time, so there is no need to verify them through electrophoresis following pcr. Description the applied biosystems 73007500 real time pcr system 73007500 system uses fluorescentbased pcr chemistries to provide quantitative detection of nucleic acid sequences using real time analysis and qualitative detection of nucleic acid sequences using endpoint and dissociationcurve analysis.
The applied biosystems stepone and steponeplus realtime pcr systems are remarkably powerful realtime pcr instruments designed with a userfriendly interface. Together with optimized qiagen kits for realtime pcr, the rotorgene q enables streamlined analysis for a wide range of applications. Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software and wizards guide new users through. Is there a maccompatible control software for the stepone rt. The abi steponeplus is an advanced real time pcr used for fast quantitative measurements.
Depending on the experimental design, the system can even help you select and order real time pcr reagents. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast and standard runs, and are configured for pcfree or networked operation. Use only optical grade tubes, flat top caps or plates, see below for order information. The steponeplus real time pcr system is a 96well real time pcr instrument perfect for both first time and experienced users. Steponeplus realtime pcr system from thermo fisher. Applied biosystems stepone realtime pcr system spectral. Smart monitoring agent for the stepone and steponeplus systems. Abi step one plus real time pcr is available from the lab world group.